Increased vulnerability to ethanol consumption in adolescent maternal separated mice
| Autor: | Adrián Bartoll, Auxiliadora Aracil, Isabel Martínez-Gras, María Salud García-Gutiérrez, Jorge Manzanares, Gabriel Rubio, José L. Lanciego, Francisco Navarrete |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Elevated plus maze Medicine (miscellaneous) Nucleus accumbens 03 medical and health sciences 0302 clinical medicine Glucocorticoid receptor Internal medicine medicine Hippocampus (mythology) Pharmacology biology Tyrosine hydroxylase Ventral tegmental area Psychiatry and Mental health 030104 developmental biology Endocrinology medicine.anatomical_structure nervous system Hypothalamus Anesthesia biology.protein NeuN Psychology 030217 neurology & neurosurgery |
| Zdroj: | Addiction Biology. 21:847-858 |
| ISSN: | 1355-6215 |
| DOI: | 10.1111/adb.12266 |
| Popis: | The purpose of this study was to evaluate the effects of early life stress on the vulnerability to ethanol consumption in adolescence. To this aim, mice were separated from their mothers for 12 hours/day on postnatal days 8 and 12. Emotional behavior (light-dark box, elevated plus maze and tail suspension tests) and pre-attentional deficit (pre-pulse inhibition) were evaluated in adolescent maternal separated (MS) mice. Alterations of the corticotropin-releasing factor (CRF), glucocorticoid receptor (NR3C1), tyrosine hydroxylase (TH), mu-opioid receptor (MOr), brain-derived neurotrophic factor (BDNF), neuronal nuclei (NeuN), microtubule-associated protein 2 (MAP2) and neurofilament heavy (NF200)-immunoreactive fibers were studied in the paraventricular nucleus of the hypothalamus (PVN), ventral tegmental area (VTA), nucleus accumbens (NAc) or hippocampus (HIP). The effects of maternal separation (alone or in combination with additional stressful stimuli) on ethanol consumption during adolescence were evaluated using the oral ethanol self-administration paradigm. MS mice presented mood-related alterations and pre-attentional deficit. Increased CRF, MOr and TH, and reduced BDNF, NR3C1, NeuN, MAP2 and NF200-immunoreactive fibers were observed in the PVN, NAc and HIP of adolescent MS mice. In the oral ethanol self-administration test, adolescent MS mice presented higher ethanol consumption and motivation. Exposure to additional new stressful stimuli during adolescence significantly increased the vulnerability to ethanol consumption induced by maternal separation. These results clearly demonstrated that exposure to early life stress increased the vulnerability to ethanol consumption, potentiated the effects of stressful stimuli exposure during adolescence on ethanol consumption and modified the expression of key targets involved in the response to stress, ethanol reinforcing properties and cognitive processes. |
| Databáze: | OpenAIRE |
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