A Simplified Branching Assay forRhizoctonia Solani
| Autor: | A. J. Downer, V. N. Armentrout, S. T. Nameth |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hyphal growth Parafilm biology Hypha Physiology Hyphal tip Cellophane Cell Biology General Medicine 030108 mycology & parasitology biology.organism_classification 010603 evolutionary biology 01 natural sciences law.invention Rhizoctonia solani 03 medical and health sciences law Botany Genetics Molecular Biology Ecology Evolution Behavior and Systematics Mycelium Plant stem |
| Zdroj: | Mycologia. 78:657-663 |
| ISSN: | 1557-2536 0027-5514 |
| DOI: | 10.1080/00275514.1986.12025302 |
| Popis: | Two different measures of branching, the Interode Length Unit (ILU) and the Hyphal Growth Unit (HGU), were applied to Rhizoctonia solani Kiihn, AG4. The ILU (the mean length in 4um of 10 internodes) was simple to perform and yielded consistent results. Results using this measure and the HGU (the mean length of mycelium per hyphal tip) as a measure of branching were compared. Growth of R. solani on media containing different carbon sources or on different concentrations of sucrose or glucose all yielded ILU of ca. 200-225 ,tm; thus branching was not affected by these treatments. Internode lengths were also constant over 29 hours for entire colonies of R. solani. The hyphae were more highly branched (mean ILU = 134 ,m) when the fungus was grown with cellophane interposed between the mycelium and the culture medium. Hyphae aligned in the grooves of scored Parafilm were more highly branched (ILU = ca. 165 ,m) than hyphae on smooth Parafilm (ILU = ca. 300 ,um); these branching frequencies were not affected by the presence of a nutrient medium. The Internode Length Unit is a simple, repeatable, and descriptive unit for measurement of lateral branch production in Rhizoctonia solani. |
| Databáze: | OpenAIRE |
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