Hybridization of Homopolynucleotides with Different Base Ordering on the Carbon Nanotube Surface
| Autor: | Valery Andrushchenko, V.S. Leontiev, Victor A. Karachevtsev, M.V. Karachevtsev, Galina O. Gladchenko |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Nanotube Materials science Kinetics Polymer Carbon nanotube Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Crystallography Molecular dynamics General Energy chemistry law Duplex (building) Homopolynucleotide Physical and Theoretical Chemistry Thermostability |
| Zdroj: | The Journal of Physical Chemistry C. 119:11991-12001 |
| ISSN: | 1932-7455 1932-7447 |
| Popis: | Hybridization of nucleic acids on the carbon nanotube is of great interest due to its potential application in base sequencing and genetic testing. In this work, we have compared the hybridization of the two complementary homopolynucleotides poly(rI) and poly(rC) on the nanotube when either the ordered poly(rC) or disordered poly(rI) was adsorbed to the nanotube surface while the complementary homopolynucleotide was free in the suspension. The hybridization of these polymers is characterized by slower kinetics of the duplex formation on the nanotube in comparison with the case of free polymer hybridization. The hybridized duplex also shows a reduced thermostability determined from UV absorption spectra upon heating. These observations point to the defects in the structure of the duplex hybridized on the nanotube. Spectroscopic measurements and molecular dynamics simulations demonstrate that the defects of the hybridized duplex are more pronounced for the case when free poly(rC) is bound to poly(rI) adsorb... |
| Databáze: | OpenAIRE |
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