Cod Larviculture Using High-density Rotifer Production with Different Enrichments
| Autor: | Benjamin S. Forward, Fabrice Pernet, Sébastien Plante, Rémy Haché |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Vibrio anguillarum animal structures genetic structures Ontogeny Rotifer Aquatic Science 03 medical and health sciences chemistry.chemical_compound 14. Life underwater Food science biology fungi 04 agricultural and veterinary sciences biology.organism_classification Eicosapentaenoic acid Fishery 030104 developmental biology chemistry Docosahexaenoic acid 040102 fisheries 0401 agriculture forestry and fisheries lipids (amino acids peptides and proteins) Arachidonic acid Docosapentaenoic acid Agronomy and Crop Science Temperature gradient gel electrophoresis |
| Zdroj: | Journal of the World Aquaculture Society. 48:57-69 |
| ISSN: | 0893-8849 |
| Popis: | High-density (HD) rotifer culture systems have been recently commercialized, but are not commonly used by the aquaculture industry. The aim of this study was to determine if HD systems could be used in cod hatcheries. An enrichment strategy using the commercial products, ArteMac and Protein Selco Plus (Com), was compared with the manufacturer's suggested enrichment, using Pavlova-DHA (Pav). The Pav enrichment increased the eicosapentaenoic acid (EPA) levels in rotifers, but reduced the docosahexaenoic acid (DHA) and 22: 5n-6 (n-6DPA, docosapentaenoic acid) levels. Larvae EPA levels in both polar and neutral lipids were relatively stable in the larvae fed with Com-rotifers; while they were higher in early stages, they were progressively reduced through ontogeny in the Pav-rotifers fed-larvae. DHA levels in polar lipids decreased in larvae, particularly when fed with HD-Pav rotifers. In all larvae, arachidonic acid (ARA) levels increased in the polar and neutral lipids, regardless of treatments. In both lipid fractions, the levels of ARA were quite stable in time, but still higher in larvae fed with Com-rotifers. Bacterial load was lower in larvae fed with Pav-rotifers. Denaturing gradient gel electrophoresis (DGGE) bacterial profiles of larvae and rotifers were all similar. This study shows the potential of usingHDsystems to produce rotifers, but highlights the necessity of adjusting the nutritional composition of rotifers prior to being fed to larvae. |
| Databáze: | OpenAIRE |
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