Development of a sequence-characterized amplified region marker using inter-simple sequence repeats for detection of Puccinia striiformis f. sp. tritici
Autor: | Shuhe Wang, Qi Liu, Bingyao Chu, Zhanhong Ma, Yong Luo |
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Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Physiology Plant Science Biology 01 natural sciences law.invention 03 medical and health sciences chemistry.chemical_compound law Genetics Pathogen Polymerase chain reaction Sequence (medicine) Inoculation fungi food and beverages Plant disease genomic DNA 030104 developmental biology chemistry Microsatellite Agronomy and Crop Science DNA 010606 plant biology & botany |
Zdroj: | Journal of Phytopathology. 165:442-447 |
ISSN: | 0931-1785 |
Popis: | Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is the most devastating wheat disease in China. Early and accurate detection of the pathogens would facilitate effective control of the diseases. DNA-based methods now provide essential tools for accurate plant disease diagnosis. In this study, inter-simple sequence repeats (ISSR) technique has been successfully applied to develop a sequence-characterized amplified region (SCAR) marker for diagnosis of stripe rust of wheat and detection of Pst. In this study, one fragment unique to Pst was identified by ISSR and then sequenced. Based on the specific fragment, a pair of SCAR primers (616AF/616AR) was designed to amplify a 299-bp DNA fragment within the sequenced region. The primers can amplify a unique DNA fragment for all tested isolates of Pst but not for the other pathogens of wheat leaves and the uninfected leaves. The polymerase chain reaction (PCR) assay could detect as low as 0.1 ng of genomic DNA in a 25.0 μl PCR reaction mixture and detect the pathogen from asymptomatic wheat leaves inoculated with Pst under glasshouse conditions. |
Databáze: | OpenAIRE |
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