Molecular diagnosis of B- and T-cell lymphomas: fundamental principles and clinical applications
| Autor: | Gregory J. Tsongalis, William N. Rezuke, Evelyn C. Abernathy |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry T cell Biochemistry (medical) Clinical Biochemistry Pcr assay Gene rearrangement medicine.disease Minimal residual disease Lymphoma law.invention Immunophenotyping medicine.anatomical_structure law medicine business Polymerase chain reaction Southern blot |
| Zdroj: | Clinical Chemistry. 43:1814-1823 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/43.10.1814 |
| Popis: | Molecular diagnostic assays have become routine in the evaluation of lymphoid malignancies. Both Southern transfer and polymerase chain reaction (PCR) technologies are used to assess for B- and T-cell clonality, the presence of rearrangements involving protooncogenes such as bcl-1 and bcl-2, and the monitoring of minimal residual disease. We review the fundamentals of B- and T-cell ontogeny as well as the basic principles of the Southern transfer and PCR assays and their applications to the diagnosis of lymphoid malignancies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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