Isolation and characterization of the cell-surface polysaccharides ofPorphyromonas gingivalisATCC 53978
| Autor: | S. I. Farquharson, G. R. Germaine, G. R. Gray |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification biology Rhamnose Immunology Mannose biology.organism_classification Polysaccharide Glucuronic acid Microbiology carbohydrates (lipids) chemistry.chemical_compound chemistry Biochemistry Galactose Extracellular Tetrasaccharide General Dentistry Porphyromonas gingivalis |
| Zdroj: | Oral Microbiology and Immunology. 15:151-157 |
| ISSN: | 0902-0055 |
| Popis: | The cell-surface carbohydrates of Porphyromonas gingivalis strain ATCC 53978 were isolated and partially characterized. Three separate polysaccharides were found to be present: an extracellular polysaccharide, capsular polysaccharide and lipopolysaccharide. The capsular polysaccharide, which had peculiar, gel-like viscoelastic properties, was found to be comprised of mannuronic acid, glucuronic acid, galacturonic acid, galactose, and 2-acetamido-2-deoxy-D-glucose in relative molar ratios of 0.6:0.9:0.5:0.5:1.0, respectively. The extracellular polysaccharide was found to contain mannose, rhamnose, glucose, galactose, and 2-acetamido-2-deoxy-D-glucose in relative molar ratios of 13.5:1.4:1.0:2.0:1.0, respectively. The lipopolysaccharide was found to contain an O-antigen with a regular tetrasaccharide repeat unit comprised of 4-linked alpha-L-rhamnopyranosyl, 6-linked alpha-D-glucopyranosyl, 3-linked alpha-D-galactopyranosyl, and 4-linked 2-acetamido-2-deoxy-beta-D-glucopyranosyl residues in equimolar proportions. |
| Databáze: | OpenAIRE |
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