The pharmacology of hSK1 Ca2+-activated K+channels expressed in mammalian cell lines
| Autor: | Mala M. Shah, D.G. Haylett |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pharmacology
Dequalinium 0303 health sciences biology Chemistry Xenopus Afterhyperpolarization Potassium channel blocker Hyperpolarization (biology) Apamin biology.organism_classification Potassium channel 3. Good health 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Slow afterhyperpolarization medicine 030217 neurology & neurosurgery 030304 developmental biology medicine.drug |
| Zdroj: | British Journal of Pharmacology. 129:627-630 |
| ISSN: | 0007-1188 |
| Popis: | The pharmacology of hSK1, a small conductance calcium-activated potassium channel, was studied in mammalian cell lines (HEK293 and COS-7). In these cell types, hSK1 forms an apamin-sensitive channel with an IC(50) for apamin of 8 nM in HEK293 cells and 12 nM in COS-7 cells. The currents in HEK293 cells were also sensitive to tubocurarine (IC(50)=23 microM), dequalinium (IC(50)=0.4 microM), and the novel dequalinium analogue, UCL1848 (IC(50)=1 nM). These results are very different from the pharmacology of hSK1 channels expressed in Xenopus oocytes and suggest the properties of the channel may depend on the expression system. Our findings also raise questions about the role of SK1 channels in generating the apamin-insensitive slow afterhyperpolarization observed in central neurones. |
| Databáze: | OpenAIRE |
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