| Autor: |
Heba Sharawy, AbdelRaouf Hegab, Engy Risha, Mohamed El Adl, Walid Soliman, Mohamed Gohar, Reham Fahmy, Virginia Farag, Kazuhiko Imakawa, Fuller Bazer, Daniela James, Adel Zaghloul, Abdelnasser Abdalla, Mariam Rabie, Mohammed Elmetwally |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-1762159/v1 |
| Popis: |
Aim OvSynch is a hormonal protocol for synchronization of estrus and use of artificial insemination (AI) at an optimal time without adverse effects on the ovaries or uterus. This study investigated the use of noninvasive color Doppler ultrasound to assess changes in uterine and vaginal blood flow during the Ovsynch program for synchronization of estrus and its relation to the pregnancy rates in Holstein cows. Materials and methods The experimental cows received an intramuscular dose of 10 µg of a GnRH analogue (G1), followed 7 days later with an intramuscular injection of synthetic prostaglandin F2α (P: PGF2α) analogue (500 µg cloprostenol sodium), and given a 10 µg, injection of the GnRH analogue (G2) i.m. (2.5ml/animal) 48 h after the PGF2a treatment, and the cows were bred 14–16 h after. Uterine and vaginal perfusion were investigated by performing transrectal Doppler ultrasonography of both the middle uterine and vaginal arteries in Holstein cows at different time points during the Ovsynch program to determine: peak systolic velocity (PSV), time-averaged maximum velocity (TAMV), the volume of blood flow (BFV), pulsatility index (PI), resistance index (RI), resistance impedance (S/D) and diameters of uterine (UA) and vaginal (VA) arteries. Steroid hormones were also considered. Transrectal ultrasonography (TUS) was performed at 32 and 60 days to confirm the pregnancy per artificial insemination (P/AI). Results The uterine PSV, TAMV, and PV were greater at the time of the cloprostenol sodium and second GnRH injections (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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