Characterization of callus formation and camptothecin production by cell lines of Camptotheca acuminata

Autor:	Marien P. Harkes, Reinetta M. Buitelaar, Arjon J. van Hengel, Paul G. M. Hesselink, Harry J. Wichers
Rok vydání:	1992
Předmět:	Callus formation Plant physiology Horticulture Biology Secondary metabolite biology.organism_classification Biochemistry Cell culture Callus Botany medicine heterocyclic compounds biological phenomena cell phenomena and immunity Secondary metabolism Nyssaceae Camptothecin medicine.drug
Zdroj:	Plant Cell, Tissue and Organ Culture. 28:11-18
ISSN:	1573-5044 0167-6857
DOI:	10.1007/bf00039910
Popis:	Camptotheca acuminata plants contain camptothecin which is a secondary metabolite with strong anti-tumor activity. The induction of callus and cell suspension cultures from Camptotheca acuminata stem parts on different media is described. Growth in the original media and in media with different salts and/or vitamins was followed by measuring several growth parameters. Camptothecin was detected and identified by means of TLC, HPLC and GC-MS. The production of camptothecin in cell suspension cultures was followed and compared to the original plant material. Results indicate that high density cultures could be easily established, producing ca 1 mg l-1 of camptothecin.
Databáze:	OpenAIRE
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