Thermal equivalence of DNA duplexes without calculation of melting temperature

Autor:	Gerald Weber, Nava E. Whiteford, Niall J. Haslam, Jonathan W. Essex, Adam Prügel-Bennett, Cameron Neylon
Rok vydání:	2005
Předmět:	Physics General Physics and Astronomy Thermodynamics DNA sequencing law.invention chemistry.chemical_compound chemistry DNA computing law DNA Microarray Analysis Helix Thermal Equivalence (measure theory) DNA Polymerase chain reaction
Zdroj:	Nature Physics. 2:55-59
ISSN:	1745-2481 1745-2473
Popis:	The common key to nearly all processes involving DNA is the hybridization and melting of the double helix: from transmission of genetic information and RNA transcription, to polymerase chain reaction and DNA microarray analysis, DNA mechanical nanodevices and DNA computing. Selecting DNA sequences with similar melting temperatures is essential for many applications in biotechnology. We show that instead of calculating these temperatures, a single parameter can be derived from a statistical-mechanics model that conveniently represents the thermodynamic equivalence of DNA sequences. This parameter is shown to order experimental melting temperatures correctly, is much more readily obtained than the melting temperature, and is easier to handle than the numerous parameters of empirical regression models.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::00aa947c58ff36a1de74b4609a0e2f27 https://doi.org/10.1038/nphys189 Zobrazit plný text záznamu