Protection against ultraviolet A-induced oxidative damage in normal human epidermal keratinocytes under post-menopausal conditions by an ultraviolet A-activated caged-iron chelator: a pilot study
| Autor: | Daniel B. Yarosh, Nadine Pernodet, Charareh Pourzand, Daniel H. Maes, Kelly Dong, Edward Pelle, Lieve Declercq, Qing Yang, Xi Huang, Jinlong Jian |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species biology Chemistry Immunology Human skin Dermatology General Medicine Metabolism Pharmacology medicine.disease_cause Desquamation Lipid peroxidation Ferritin chemistry.chemical_compound medicine.anatomical_structure Biochemistry medicine biology.protein Immunology and Allergy Radiology Nuclear Medicine and imaging sense organs medicine.symptom Keratinocyte Oxidative stress |
| Zdroj: | Photodermatology, Photoimmunology & Photomedicine. 27:231-235 |
| ISSN: | 0905-4383 |
| DOI: | 10.1111/j.1600-0781.2011.00604.x |
| Popis: | Background/purpose: Human skin is constantly exposed to ultraviolet A (UVA), which can generate reactive oxygen species and cause iron release from ferritin, leading to oxidative damage in biomolecules. This is particularly true in post-menopausal skin due to an increase in iron as a result of menopause. As iron is generally released through desquamation, the skin becomes a main portal for the release of excess iron in this age group. In the present study, we examined a strategy for controlling UVA- and iron-induced oxidative stress in skin using a keratinocyte post-menopausal cellular model system. Methods: Keratinocytes that had been cultured under normal or high-iron, low-estrogen conditions were treated with (2-nitrophenyl) ethyl pyridoxal isonicotinoyl hydrazone (2-PNE-PIH). 2-PNE-PIH is a caged-iron chelator that does not normally bind iron but can be activated by UVA radiation to bind iron. Following incubation with 2-PNE-PIH, the cells were exposed to 5 J/cm2 UVA and then measured for changes in lipid peroxidation and ferritin levels. Results: 2-PNE-PIH protected keratinocytes against UVA-induced lipid peroxidation and ferritin depletion. Further, 2-PNE-PIH was neither cytotoxic nor did it alter iron metabolism. Conclusion: 2-PNE-PIH may be a useful deterrent against UVA-induced oxidative stress in post-menopausal women. |
| Databáze: | OpenAIRE |
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