High performance liquid chromatography hyphenated to inductively coupled plasma mass spectrometry for V and Ni quantification as tetrapyrroles
| Autor: | Tatiana D. Saint'Pierre, Norbert Miekeley, Christiane B. Duyck, Peter Szatmari, Teresa C.O. Fonseca |
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| Rok vydání: | 2011 |
| Předmět: |
Detection limit
Chloroform Chromatography Elution Analytical chemistry Fraction (chemistry) Reversed-phase chromatography High-performance liquid chromatography Atomic and Molecular Physics and Optics Analytical Chemistry chemistry.chemical_compound chemistry Methanol Instrumentation Inductively coupled plasma mass spectrometry Spectroscopy |
| Zdroj: | Spectrochimica Acta Part B: Atomic Spectroscopy. 66:362-367 |
| ISSN: | 0584-8547 |
| Popis: | A method was developed for the determination of V and Ni as tetrapyrroles by High Performance Liquid Chromatography hyphenated to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP-MS) using reversed phase and elution gradient. Chlorinated solvents and tetrahydrofuran were investigated as regard to separation time and ICP-MS detection efficiencies. The final elution gradient program started from pure methanol to a mixture of 20:80 (v/v) chloroform:methanol. External quantification of V and Ni with inorganic standards by flow injection ICP-MS, used online with HPLC, resulted in 95% of recoveries. The Limits of Detection for V during methanol elution and for Ni during the 20% chloroform gradient elution were evaluated by their minimum detectable concentrations, which were, respectively, 5 μg L− 1 and 8 μg L− 1. The methodology was applied to polar and resin fractions separated from a Brazilian crude oil and a sediment extract from an oil-polluted area in the Guanabara Bay, Rio de Janeiro, Brazil. Vanadium as tetrapyrroles represented the totality of V content in the polar fraction, whereas Ni was in different polar forms in the resin and sediment extract. |
| Databáze: | OpenAIRE |
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