Hepatic AA amyloidosis in a cat: cytologic and histologic identification of AA amyloid in macrophages
| Autor: | Sakurako Neo-suzuki, Yukako Mochizuki, Masaharu Hisasue, Hideki Kayanuma, Takayuki Mineshige, Ryo Tsuchiya, Junichi Kamiie, Kinji Shirota |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Pathology General Veterinary Amyloid 040301 veterinary sciences business.industry Amyloidosis 04 agricultural and veterinary sciences 030204 cardiovascular system & hematology medicine.disease Stain Staining 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Perisinusoidal space AA amyloidosis medicine Histopathology Serum amyloid A business |
| Zdroj: | Veterinary Clinical Pathology. 46:331-336 |
| ISSN: | 0275-6382 |
| Popis: | A 3-year-old, spayed female, Domestic Shorthair cat presented with anorexia, lethargy, vomiting, probable hemoabdomen, and multiple masses on the right lateral liver lobe. Clinicopathologic and imaging abnormalities included anemia, azotemia, icterus, and hepatomegaly with hypoechoic masses. On cytologic evaluation of a fine-needle aspiration of a liver mass there was abundant extracellular pink- to purple-colored material between hepatocytes. The amorphous material was stained with direct fast scarlet (DFS), and green birefringent areas were observed under polarized light, confirming the presence of amyloid. A unique finding on the cytologic smear were macrophages containing amorphous and fibrillar amyloid-like protein. Histopathologic examination using H&E and Congo red staining confirmed amyloid deposits within the space of Disse, along the sinusoids, portal tracts, blood vessel walls, and within the cytoplasm of macrophages. Immunohistochemical staining with anti-AA amyloid antibodies further confirmed the presence of AA amyloid. To the author's knowledge, this is the first report of the cytologic finding of AA amyloid protein within macrophages and DFS stain detection of amyloid on a cytologic smear. |
| Databáze: | OpenAIRE |
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