Eine Methode zur Quantitativen Verfolgung von Festphasenreaktionen in der Peptidsynthese

Autor:	Frank Raddatz, Klaus‐Dieter Kaufmann
Rok vydání:	1978
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Chromatography chemistry Sephadex Elution Phase (matter) Peptide synthesis Dimethylformamide Peptide Hydrazide Pentapeptide repeat
Zdroj:	Journal f�r Praktische Chemie. 320:847-856
ISSN:	1521-3897 0021-8383
DOI:	10.1002/prac.19783200518
Popis:	A Method for the Quantitative Control of Solid Phase Reactions in the Peptide Synthesis A quantitative control-method of partial steps of a solid phase peptide synthesis is described, based on a hydrazinolytic cleavage of protected peptides from the solid support. A separation of the peptide hydrazides by gelchromatography on sephadex G 15 in dimethylformamide and a quantitative evaluation of the hydrazide-content in the elution fractions is reported, which permits the control of the Nα-deprotection step in the same way as the evaluation of the coupling yields of the desired sequence. The new method is discussed for the example of two solid phase syntheses of the pentapeptide N-tert.-butyl-oxycarbonyl-L-valyl-S-benzyl-L-cysteinyl-L-alanyl-L- leucyl-O-benzyl-L-tyrosine hydrazide.
Databáze:	OpenAIRE
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