Aggregationsstufen und Biosyntheseleistungen von Rattenleber-Ribosomen unter dem Einfluß Ribosomen-spezifischer Antikörper

Autor:	Jürgen G. Meyer-Bertenrath, Hannelore Würz
Rok vydání:	1967
Předmět:	Antiserum Hemagglutination biology Cytoplasm Chemistry biology.protein General Chemistry Plasma protein binding Ribosomal RNA Binding site Antibody Molecular biology Ribosome
Zdroj:	Zeitschrift für Naturforschung B. 22:1153-1156
ISSN:	1865-7117 0932-0776
DOI:	10.1515/znb-1967-1113
Popis:	The formation of antibodies against rat liver ribosomes was induced by intravenous injections of ribosomal preparations into sheep and rabbits. These preparations did not contain any cytoplasmic proteins detectable by immunoelectrophoresis. The existence of specific antibodies was demonstrated by precipitation, haemagglutination and complement fixation. During incubation of ribosomes with antiserum an aggregation of the ribosomes takes place. Obviously, the formation of dimeric aggregates is a direct antigen-antibody-reaction, which, in a secondary process, may be followed by formation af heavier aggregates, containing at least six ribosomes. These complexes are no longer active in synthetizing proteins in vitro. It may be concluded, therefore, that on the surface of the ribosome the site of antibody fixation is located closely to the protein binding site and the transfer-RNA binding site, respectively.
Databáze:	OpenAIRE
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