Phase separation within artificial granules from a blend of polyhydroxybutyrate and polyhydroxyoctanoate: biological implications
| Autor: | Jeremy K. M. Sanders, Daniel M. Horowitz |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
education.field_of_study Materials science Polymers and Plastics Density gradient Organic Chemistry Granule (cell biology) Population Polymer Elastomer Miscibility Polyhydroxybutyrate Polyester chemistry Chemical engineering Polymer chemistry Materials Chemistry education |
| Zdroj: | Polymer. 35:5079-5083 |
| ISSN: | 0032-3861 |
| DOI: | 10.1016/0032-3861(94)90668-8 |
| Popis: | A recently developed in vitro procedure for preparing artificial granules of microbial polyesters has been applied to a blend of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxyoctanoate) (PHO). Density gradient analysis showed that these granules formed a single, uniform population; n.m.r. showed that the polymers retained their amorphous elastomeric state; and thermal analysis indicated that the polymers formed separate phases within these granules. These results contrast strongly with the observation that a recombinant bacterial strain simultaneously expressing PHB and PHO stores the two polymers in separate cellular granules. The implications of the in vitro findings for granule formation in vivo are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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