NIR studies of cholesterol-dependent structural modification of the model lipid bilayer doped with inhalation anesthetics
| Autor: | Katarzyna Cieślik-Boczula, Maria Rospenk, Marta Kuć |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030103 biophysics Cholesterol Organic Chemistry Enflurane 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Membrane chemistry Isoflurane Dipalmitoylphosphatidylcholine medicine Physical chemistry lipids (amino acids peptides and proteins) Halothane Lipid bilayer Spectroscopy medicine.drug |
| Zdroj: | Journal of Molecular Structure. 1162:17-25 |
| ISSN: | 0022-2860 |
| DOI: | 10.1016/j.molstruc.2018.02.086 |
| Popis: | The influence of cholesterol on the structure of the model lipid bilayers treated with inhalation anesthetics (enflurane, isoflurane, sevoflurane and halothane) was investigated employing near-infrared (NIR) spectroscopy combined with the Principal Component Analysis (PCA). The conformational changes occurring in the hydrophobic area of the lipid bilayers were analyzed using the first overtones of symmetric (2νs) and antisymmetric (2νas) stretching vibrations of the CH2 groups of lipid aliphatic chains. The temperature values of chain-melting phase transition (Tm) of anesthetic-mixed dipalmitoylphosphatidylcholine (DPPC)/cholesterol and dipalmitoylphosphatidylglycerol (DPPG)/cholesterol membranes, which were obtained from the PCA analysis, were compared with cholesterol-free DPPC and DPPG bilayers mixed with inhalation anesthetics. |
| Databáze: | OpenAIRE |
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