Rapid Decrease in DNA Content Induced by Gamma Irradiation in Cultured Cells of Tobacco

Autor:	Mitsuhiro Hatanaka, Akira Kanazawa, Atsushi Hirai, Hoshio Eguchi, Nobuhiro Tsutsumi
Rok vydání:	1998
Předmět:	Radiation biology fungi biology.organism_classification chemistry.chemical_compound chemistry Biochemistry Cell culture Magnoliopsida Nucleic acid Growth rate Irradiation Incubation DNA Nicotiana
Zdroj:	RADIOISOTOPES. 47:399-404
ISSN:	1884-4111 0033-8303
DOI:	10.3769/radioisotopes.47.399
Popis:	The effect of gamma irradiation on the growth rate, on the amount of soluble protein and on the amount of DNA in tobacco calli was analyzed. A significant decrease in the amount of DNA was detected in the calli of every growth stage after incubation for 24hfollowing irradiation at a dose that severely affected the growth of calli, although only a slight change was detected in the amount of soluble protein. No difference in the effect of irradiation on protein and DNA contents was detected among calli of different growth stages, although the amounts of protein and DNA in calli in each growth stage were different. The extent of the decrease in the amount of DNA of calli depended on both the dose which ranged from 0-300Gyand the time of incubation after irradiation. These results suggest that the rapid decrease in the DNA content of calli after irradiation is due to cellular systems that are induced by irradiation and that result in enzymatic degradation of DNA.
Databáze:	OpenAIRE
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