| Autor: |
Amina A. Radwan, Mahmoud L. Sayed, Mohamed A. Abdrabo, Nourhan Nagy, Anhar A. Abdel Latef, Samah E. Abo Dalal, Afaf A. Khedr |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Zagazig Veterinary Journal. 44:149-155 |
| ISSN: |
2357-075X |
| Popis: |
Pigeon paramyxovirus type 1 (PPMV-1) and salmonellosis are two of the major health problems that affect pigeons worldwide. Immuno-protection of pigeons against PPMV-1 infection and salmonellosis is a very important preventive measure. In the present work, a combined inactivated montanide ISA-206 oil adjuvanted vaccine of local isolates of PPMV-1 and S. Typhimurium was prepared. Quality control assessment of such preparation revealed that it is free from foreign contaminants, safe and immunogenic. The sero-evaluation using microplate agglutination test revealed that the humoral immune response developed against S. Typhimurium in the vaccinated pigeons reached 64 three weeks post 1 st dose and reached its maximum value 256 two weeks post boostering. Results of HI test showed that the vaccine induced detectable humoral immune response to PPMV-1 expressed by marked increased HI antibody titer till the end of the experiment (8.0 log 2 ). The vaccination-challenge assay with the virulent strain of PPMV-1 with 10 6 EID 50 /mL showed 100% protection in vaccinated group (Ia). While, the virulent S. Typhimurium organism with 5 x10 7 CFU/bird showed 90% protection in vaccinated birds. The unvaccinated control group showed 10% and 20% protection against both virulent PPMV-1 and S . Typhimurium, respectively. In conclusion, this vaccine could be recommended as safe, potent and could be useful when used for protection against PPMV-1 and S. Typhimurium under field condition. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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