| Popis: |
Marine macroalgae are nutraceuticals rich in nutritional profile and secondary bioactive metabolites. However, they have varied nutritional and biochemical qualities due to a variety of reasons. This study aimed to determine the phytochemical profile and biological activities of Caulerpa racemosa, edible green algae also known as Sea grapes. The study successfully identified secondary metabolites through metabolomic profiling untargeted by LC-HRMS as well as a bioactive peptide. In addition, antioxidant activity and cytotoxicity of extracts and compounds were determined. A total of 103 metabolites were identified in C. racemosa extract obtained by the maceration, while 48 were detected in the soxhlet extract. A peptide with the sequence ELWKTF (Glu-Leu-Trp-Lys-Thr-Phe; C41H58N8O) and its abundance was identified in the α-chymotrypsin hydrolysate of C. racemosa. In the antioxidant activity test, Soxhletated-extract (ES) and purified fraction 1 (PF1) had half-maximal effective concentration (EC50) < EC50 of control/GSH (DPPH inhibition) and PF1 had EC50 < EC50 of control/Trolox (ABTS inhibition). The cytotoxicity results showed that macerated-extract (EM), ES, and PF1 as antioxidant agents in the observed EC50 were safe. In general, C. racemosa contains antioxidant nutrients, metabolites, and bioactive peptides, a factor that makes it a promising functional food and pharmaceutical. |
