Comparative Assessment of Toxoplasma gondii Infection Prevalence in Romania Using 3 Serological Methods
| Autor: | Adriana Györke, Anamaria Ioana Paştiu, Vasile Cozma, Anamaria Balea, Emoke Pall, Mihaela Niculae, Isabelle Villena, Marina Spinu |
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| Rok vydání: | 2015 |
| Předmět: |
biology
Prevalence Pharmaceutical Science Toxoplasma gondii medicine.disease biology.organism_classification Virology Toxoplasmosis Serology Complementary and alternative medicine Direct agglutination test parasitic diseases biology.protein medicine Potency Seroprevalence Pharmacology (medical) Antibody |
| Zdroj: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine. 72 |
| ISSN: | 1843-5378 1843-5270 |
| DOI: | 10.15835/buasvmcn-vm:10968 |
| Popis: | The aim of this study was to evaluate three serological tests used for T. gondii antibodies detection in horses. A commercially available ELISA test (ID Screen Toxoplasmosis Indirect Multi-Species, ID.vet, Innovative Diagnostics, France) and two ‘in house’ methods, indirect immunofluorescent antibody test (IFAT), modified agglutination test (MAT) were compared for their sensitivity and specificity in detection of anti-T. gondii antibodies in naturally infected horses using as standard cumulative seropositivity. Also, their agreement was evaluated by kappa statistic. A good correspondence of test results was observed. The best agreement was seen between ELISA and MAT (k=0.77). ELISA has the higher sensitivity (96.8%) and also the higher specificity (96.1%), followed by MAT (90.3 and 94.1, respectively) both tests proving better diagnostic potency compared with IFAT. The highest seroprevalence was obtained by ELISA (39%), followed by MAT (37.8%) and IFAT (34.1%). Based on our results, we recommend the utilization of indirect ELISA and MAT for detection of T. gondii antibodies in horses. |
| Databáze: | OpenAIRE |
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