| Autor: |
K Peppel, Corrado Baglioni |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Journal of Biological Chemistry. 266:6663-6666 |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(20)89546-5 |
| Popis: |
The pathway of degradation of human interferon-beta (IFN-beta) mRNA was examined in murine C127 cells that carry an expression vector for this mRNA. The IFN-beta mRNA decayed with a half-life of approximately 45 min in actinomycin D-treated cells and became gradually shorter. This mRNA was superinduced in cycloheximide-treated cells, but it also became gradually shorter. However, apparently full-length species of IFN-beta mRNA accumulated after prolonged incubation with cycloheximide. The shortened IFN-beta mRNA species were partially deadenylated and less stable than full-length species. These findings suggest that at least two nuclease activities are involved in degrading IFN-beta mRNA; one deadenylates this mRNA and decays in cycloheximide-treated cells, while the other apparently breaks down deadenylated mRNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
