A Simple Nano-SiO2-Based ELISA Method for Residue Detection of 2,4-Dichlorophenoxyacetic Acid in Bean Sprouts
| Autor: | Lin-Sen Qing, Jie Li, Xing-De Li, Jing Xie, Qian-Long Wang, Li-Sheng Ding |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Residue (complex analysis) Chromatography 2 4-Dichlorophenoxyacetic acid biology 010401 analytical chemistry Nano sio2 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Applied Microbiology and Biotechnology Horseradish peroxidase 0104 chemical sciences Analytical Chemistry Highly sensitive chemistry.chemical_compound Linear range chemistry biology.protein Elisa method 0210 nano-technology Safety Risk Reliability and Quality Safety Research Food Science |
| Zdroj: | Food Analytical Methods. 10:1500-1506 |
| ISSN: | 1936-976X 1936-9751 |
| Popis: | The abusive use of 2,4-dichlorophenoxyacetic acid (2,4-D) was very common in the commercial production of bean sprouts. Herein, a simple and highly sensitive nano-SiO2-based ELISA method for residual 2,4-D detection was developed. The test depends on the direct competitive combination to anti-2,4-D antibody labeled by horseradish peroxidase (HRP) between nano-SiO2 immobilized by ovalbumin-2,4-D (SiO2-OVA-2,4-D) and free 2,4-D in sample. The linear range of this method was from 1 to 350 ng/mL (r = 0.997), and the limit of detection (LOD) was 0.079 ng/mL. The coefficient variations of intra-day and inter-day were all lower than 10% at three concentrations (20, 150, and 250 ng/mL). The mean recovery was 93.2%, and the specificity was satisfactory. The results indicated that the method could be useful for fast determination of residual 2,4-D in bean sprout samples using classic TMP/HRP system and a conventional UV spectrophotometer. |
| Databáze: | OpenAIRE |
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