| Autor: |
Jay F. Sarthy, Suzan Imren, Brandon Hadland, Soheil Meshinchi, Sommer Castro, Keith R. Loeb, Benjamin J. Huang, Cynthia Nourigat, Scott N. Furlan, LaKeisha Perkins, Shivani Srivastava, Rhonda E. Ries, Thao T Tang, Irwin D. Bernstein, Jenny L. Smith, Lisa Eidenschink Brodersen, Katherine Tarlock, Stanley R. Riddell, Larua Pardo, Lindsey F Call, Amy Beckman, Rhonda Idemmili, Amanda R. Leonti, Tiffany A. Hylkema, Takashi Ishida, Anisha M Loeb, Quy Le, Loken Michael, Robin Williams |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Fusion oncoproteins are the initiating event in AML pathogenesis, although they are thought to require additional cooperating mutations for leukemic transformation. CBFA2T3-GLIS2 (C/G) fusion occurs exclusively in infants and is associated with highly aggressive disease1-4. Here we report that lentiviral transduction of C/G fusion is sufficient to induce malignant transformation of human cord blood hematopoietic stem and progenitor cells (CB HSPCs) that fully recapitulates C/G AML. Engineered CB HSPCs co-cultured with endothelial cells undergo complete malignant transformation with identical molecular, morphologic, phenotypic and disease characteristics observed in primary C/G AML. Interrogating the transcriptome of engineered cells identified a library of C/G fusion-specific targets that are candidates for chimeric antigen receptor (CAR) T cell therapy. We developed CAR-T cells directed against one of the targets, FOLR1, and demonstrated the pre-clinical efficacy against C/G AML while sparing normal hematopoiesis. Our findings underscore the role of the endothelial niche in promoting leukemic transformation of C/G-transduced CB HSPCs. Moreover, this work has broad implications for studies of leukemogenesis applicable to a variety of oncogenic fusion-driven pediatric leukemias, providing a robust and tractable model system to characterize the molecular mechanisms of leukemogenesis and identify biomarkers for disease diagnosis and targets for therapy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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