A novel strain of Bacillus amyloliquefaciens displaying broad spectrum antifungal activity and its underlying mechanism
| Autor: | Satyendra Gautam, Sachin N. Hajare, Arun Sharma |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Bacillus (shape) Bacillus amyloliquefaciens biology Strain (chemistry) Sequence analysis Fatty acid Lipopeptide biology.organism_classification Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Bacillomycin chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry Pulsed-field gel electrophoresis |
| Zdroj: | Annals of Microbiology. 66:407-416 |
| ISSN: | 1869-2044 1590-4261 |
| DOI: | 10.1007/s13213-015-1123-0 |
| Popis: | One isolate among the various bacterial soil isolates screened in our study showed broad spectrum and profound antifungal activity and was characterized by biochemical and molecular approaches. Sequence analysis of the 16S rRNA gene indicated more than 98 % sequence similarity with Bacillus amyloliquefaciens ATCC 23350T and its variant Bacillus velezensis BCRC 17467T. However, the biochemical characteristics of the isolated strain, such as cellular fatty acid composition, differed significantly from those of these two Bacillus strains, with the isolated strain containing anteiso-C15:0 (terminally branched saturated fatty acids) as a predominant fatty acid. The band pattern of the pulsed field gel electrophoresis profile of AvrII-digested genomic DNA of the isolate also showed significant variation from those of the two closely related Bacillus type strains. Based on these differences, the isolate was considered to be a new strain of B. amyloliquefaciens and deposited as Bacillus amyloliquefaciens strain fiply 3A in the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) culture collection (DSM 22646) and in MTCC, IMTECH, Chandigarh (DSM 22646). Purification of the extracellular antifungal compound produced by the isolate by HPLC and its analysis by LC-ESI-MS revealed it to be a bacillomycin D-like cyclic lipopeptide. The antifungal activity of the compound was found to be due to its inhibitory effect on β-1,3-glucan biosynthesis, a major fungal cell-wall component. Thus, we describe here the identification and characterization of a novel strain of Bacillus amyloliquefaciens (fiply 3A) which produces antifungal lipopeptide and the deciphering of its mechanism of action. |
| Databáze: | OpenAIRE |
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