In Vitro Effects of Nitrogen Dioxide on the Release of Superoxide Anions, Tnf-α, and IL-8 by HL60-Macrophages and Bovine Alveolar Macrophages
| Autor: | Gerhard Polzer, Evelyn Krüger, Arnulf Seidel, Ines Lind |
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| Rok vydání: | 1994 |
| Předmět: | |
| Zdroj: | Inhalation Toxicology. 6:359-377 |
| ISSN: | 1091-7691 0895-8378 |
| DOI: | 10.3109/08958379409003033 |
| Popis: | This study was designed to investigate the in vitro effect of nitrogen dioxide on some immunofunctions of immune-competent cells, HL60-cells, differentiated into macro-phages with calcitriol (HL60-M), and bovine alveolar macrophages (BAM) were exposed to synthetic air or to NO2 (1.5 and 3.0 ppm) for 1 or 2 h. Under the special conditions of exposure, which allow the cells to be kept in direct contact with the gases during the whole incubation period, the cells stay alive in synthetic air for more than 4 h. After a lag phase of about 1 h, the exposure to NO2 results in a decrease of the viability, which is dependent both on the time and on the concentration. The morphology of the cell surface structures is obviously changed, even under conditions in which a loss of viability cannot yet be observed. Simultaneously, the capability of the cells to generate superoxide anions in response to a stimulation with zymosan, but not with TPA, is markedly reduced and, in addition, the spontaneous release of sup... |
| Databáze: | OpenAIRE |
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