| Autor: |
Tobias Knaute, Florian Toepert, Jens Schneider-Mergener, Stefan Guffler |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Peptides: The Wave of the Future ISBN: 9789401039055 |
| DOI: |
10.1007/978-94-010-0464-0_95 |
| Popis: |
We present a novel approach for the construction of large arrays of synthetic protein domains. A combination of SPOT synthesis [1] and native chemical ligation [2] was applied to generate 6859 variants of the hYAP WW protein domain [3,4] consisting of 38 amino acids. Leucine 30, histidine 32 and glutamine 35 were substituted by all other L-amino acids (excluding cysteine) resulting in 19 × 19 × 19 = 6859 variants. The C-terminal parts of the protein domains were prepared by SPOT synthesis creating 6859 C-terminal peptides comprising 24 residues with variations in the positions described above and an N-terminal cysteine. Full length protein domains were assembled upon ligation of a thioester-peptide comprising the remaining 14 residues. The ligation site (originally a serine) had been identified in previous studies [5]. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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