Spectral-luminescent study of coumarin 35 as fluorescent 'light-up' probe for BSA and DNA monitoring
| Autor: | Yavuz Onganer, Tuğba Bayraktutan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
inorganic chemicals
biology Process Chemistry and Technology General Chemical Engineering Metal ions in aqueous solution Analytical chemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Coumarin 01 natural sciences Binding constant 0104 chemical sciences Metal chemistry.chemical_compound Fluorescent light chemistry visual_art biology.protein visual_art.visual_art_medium Bovine serum albumin 0210 nano-technology Luminescence DNA Nuclear chemistry |
| Zdroj: | Dyes and Pigments. 142:62-68 |
| ISSN: | 0143-7208 |
| DOI: | 10.1016/j.dyepig.2017.03.019 |
| Popis: | The monitoring of bovine serum albumin (BSA) and deoxyribonucleic acid (DNA) in physiological pH were studied comparatively by using coumarin 35 dye (C35). For this aim, the binding constant and binding number sites for the interactions of the dye with BSA and DNA were determined by Scatchard method. According to the spectroscopic data, fluorescence intensity of C35 was 30-fold and 8.5-fold increased with the presence of BSA and DNA, respectively. Effects of metal ions on the interactions between BSA-C35 and DNA-C35 systems were also studied in terms of metal ion sensor properties. Therefore, the effect of thirteen cations (K+, Li+, NH4+,Ca2+, Ba2+, Mg2+, Sr2+, Mn2+, Fe2+, Ni2+, Al3+, Fe3+, Sn4+) on the BSA-C35 and DNA-C35 systems were investigated, the variations of binding constants were determined and comparisons were made. With this respect, this study is important in terms of gaining a new light-up probe to the literature. |
| Databáze: | OpenAIRE |
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