Transcriptional Mapping of the Coat Protein Gene of Tomato Golden Mosaic Virus

Autor:	Robert H.A. Coutts, K. W. Buck, I. T. D. Petty
Rok vydání:	1988
Předmět:	Genetics biology Polyadenylation viruses fungi food and beverages Nicotiana benthamiana Promoter biology.organism_classification Virology Molecular biology Primer extension Virus Restriction map Transcription (biology) Gene
Zdroj:	Journal of General Virology. 69:1359-1365
ISSN:	1465-2099 0022-1317
DOI:	10.1099/0022-1317-69-6-1359
Popis:	Summary Polyadenylated RNA was isolated from Nicotiana benthamiana plants infected with tomato golden mosaic virus (TGMV). Northern hybridization with a strand-specific probe of cloned TGMV DNA A revealed a 0.9 kb transcript with the same orientation as virion DNA. The positions of the 5′ and 3′ termini of the transcript, which were mapped by nuclease protection and primer extension techniques, indicated that it corresponded to the virus coat protein mRNA and enabled the likely functional promoter and polyadenylation signals to be identified.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::003bac0c5e8cacac9af250fb0e1ad8fd https://doi.org/10.1099/0022-1317-69-6-1359 Zobrazit plný text záznamu