A Novel and Reproducible Mouse Model of Spinal Cord Ischemia/Reperfusion Injury
| Autor: | Joel Linden, David Bumpass, John A. Kern, Christopher I. Shaffrey, René Roy, Stuart S. Berr, Yuesheng Li, Yuan-Ji Day, Rod J. Oskouian |
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| Rok vydání: | 2006 |
| Předmět: |
Cord
medicine.diagnostic_test business.industry medicine.medical_treatment Rehabilitation Autophagia Ischemia Laminectomy Physical Therapy Sports Therapy and Rehabilitation Magnetic resonance imaging medicine.disease Spinal cord compression Anesthesia medicine Neurology (clinical) business Reperfusion injury Spinal cord injury |
| Zdroj: | Topics in Spinal Cord Injury Rehabilitation. 12:1-10 |
| ISSN: | 1082-0744 |
| Popis: | Background: To apply the power of mice genetics to the study of spinal cord injury (SCI), improved small animal injury models are needed. We describe a novel ischemia/reperfusion murine model that is induced by spinal cord compression that can be reproduced effectively. Method: Mice were subjected to injury by placing a 10, 15, or 20 g weight on a 2 × 1 mm region of the cord at T12 for 5-15 min after laminectomy. The injury was monitored by mouse multimodality magnetic resonance imaging (MRI). The effect of changing the orientation of a 15 g weight (7.5 g/mm2) to produce a narrow injury (with the long 2 mm axis of the weight parallel to the cord) to a transverse orientation markedly influenced resulting injury. Locomotor assessments were made using the mouse BBB (mBBB) and the transformed BBB (tBBB) scoring systems. Results: A weight of 7.5 g/mm2 produced moderate injury as assessed by MRI. A large fraction of mice with transverse injury exhibited autophagia with severe infection and required euthanasia. ... |
| Databáze: | OpenAIRE |
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