Effect of rosR Gene Overexpression on Biofilm Formation by Rhizobium leguminosarum
| Autor: | A.M. Lavina, An. Kh. Baymiev, Z. R. Vershinina, L.R. Khakimova, L. R. Karimova, Al. Kh. Baymiev, Yu. M. Nikonorov, O. V. Chubukova |
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| Rok vydání: | 2021 |
| Předmět: |
Regulation of gene expression
0303 health sciences Proteases biology Endosymbiosis 030306 microbiology Chemistry Biofilm food and beverages Heterologous biochemical phenomena metabolism and nutrition medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Microbiology Rhizobium leguminosarum Rhizobia 03 medical and health sciences medicine Gene 030304 developmental biology |
| Zdroj: | Microbiology. 90:198-209 |
| ISSN: | 1608-3237 0026-2617 |
| Popis: | The regulatory protein encoded by the rosR gene is involved in the processes of adaptation of root nodule bacteria Rhizobium leguminosarum to changes in environmental conditions. It affects the expression of a number of genes associated with the synthesis of exopolysaccharides, which play a critical role in formation of bacterial biofilms on various surfaces. The goal of this work was to study the effect of overexpression of the rosR gene on formation of R. leguminosarum biofilms on inert surfaces and roots of heterologous plant hosts by obtaining recombinant strains with an additional copy of the rosR gene under the control of the Pm promoter. Our analysis of recombinant strains showed that additional expressed copies of the rosR gene allowed rhizobia to overcome the inhibitory effect on biofilm formation caused by low calcium concentrations in the medium, the effect of proteases and detergents, and to form biofilms more efficiently on the surface of tomato and clover roots. The results obtained open up prospects for using rosR as a tool both for increasing the efficiency of endosymbiosis of rhizobia with legumes, and for the formation of stable associative interactions with other agricultural crops. |
| Databáze: | OpenAIRE |
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