Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae
| Autor: | Yona Kassir, David T Stuart |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics biology ved/biology fungi Saccharomyces cerevisiae ved/biology.organism_classification_rank.species biology.organism_classification Yeast Chromosome segregation 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Meiosis Ploidy Homologous recombination Model organism Gene 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493963386 |
| DOI: | 10.1007/978-1-4939-6340-9_1 |
| Popis: | The budding yeast Saccharomyces cerevisiae has a long history as a model organism for studies of meiosis and the cell cycle. The popularity of this yeast as a model is in large part due to the variety of genetic and cytological approaches that can be effectively performed with the cells. Cultures of the cells can be induced to synchronously progress through meiosis and sporulation allowing large-scale gene expression and biochemical studies to be performed. Additionally, the spore tetrads resulting from meiosis make it possible to characterize the haploid products of meiosis allowing investigation of meiotic recombination and chromosome segregation. Here we describe genetic methods for analysis progression of S. cerevisiae through meiosis and sporulation with an emphasis on strategies for the genetic analysis of regulators of meiosis-specific genes. |
| Databáze: | OpenAIRE |
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