The psychrotrophic yeastSporobolomyces roseusLOCK 1119 as a source of a highly active aspartic protease for thein vitroproduction of antioxidant peptides
| Autor: | Marianna Turkiewicz, Joanna Krysiak, Katarzyna M. Szulczewska, Tomasz Florczak, Aneta Białkowska, Marta Wanarska |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment 030106 microbiology Biomedical Engineering Bioengineering Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Aspartic Acid Proteases Casein Drug Discovery medicine chemistry.chemical_classification Protease Chemistry Process Chemistry and Technology Enzyme biosynthesis General Medicine Yeast 030104 developmental biology Enzyme Biochemistry Molecular Medicine Specific activity Pepstatin Biotechnology |
| Zdroj: | Biotechnology and Applied Biochemistry. 65:726-738 |
| ISSN: | 0885-4513 |
| Popis: | A psychrotrophic yeast strain producing a cold-adapted protease at low temperature was classified as Sporobolomyces roseus. In standard YPG medium, S. roseus LOCK 1119 synthesized an extracellular protease with an activity of approximately 560 U/L. Optimization of medium composition and process temperature considerably enhanced enzyme biosynthesis; an approximate 70% increase in activity (2060 U/L). The native enzyme was purified to homogeneity by cation exchange chromatography followed by a size exclusion step, resulting in a 103-fold increase in specific activity (660 U/mg) with 25% recovery. The enzyme displayed 10%-30% of its maximum activity at 0-25 °C, with the optimum temperature being 50°C. Protease G8 was strongly inactivated by pepstatin A, an aspartic protease inhibitor. The enzyme was used to hydrolyze four natural substrates, and their antioxidant activities were evaluated against 1,1-diphenyl-2-picrylhydrazyl. The highest antioxidant activity (69%) was recorded for beef casein. |
| Databáze: | OpenAIRE |
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