The Effect of Mango Mistletoes (Dendrophthoe pentandra) Leaves Extract on Percentage of CD4+CD28+, CD8+CD28+, and interleukin-2 Levels of Aged Balb/c Mice
| Autor: | Syaiful Hidayat, Inmas Andi Sermoati, Muhammad Badrus Solikhin, Kusworini Handono, Yahya Irwanto, Eviana Norahmawati, Maria Gabriela Yuniati, Agustina Tri Endharti, Mirza Zaka Pratama, Ni Putu Sri Haryati |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Interleukin 2 Traditional medicine biology business.industry Spleen General Medicine Immunosenescence biology.organism_classification medicine.disease Proinflammatory cytokine BALB/c 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Immune system 030220 oncology & carcinogenesis Diabetes mellitus Medicine business Receptor medicine.drug |
| Zdroj: | Open Access Macedonian Journal of Medical Sciences. 9:414-421 |
| ISSN: | 1857-9655 |
| Popis: | BACKGROUND: Population aging is considered to be a global phenomenon today. Age-associated immune system dysfunction or “immunosenescence” is indicated by increased susceptibility to infections and chronic diseases, such as hypertension, diabetes mellitus, autoimmune diseases, heart disease, and atherosclerosis. One of the immunosenescence markers is a significant drop in CD28 and reduced proinflammatory cytokine interleukin-2 (IL-2). The mango mistletoes are deemed to have a better affinity for docking the CD28 and IL-2R receptors of α and β subunits than other plants. AIM: This study aims to determine the effect of ethanol extract of mango mistletoes on IL-2 levels, the percentage of CD4+CD28+, and the percentage of CD8+CD28+ in aged female mice. METHODS: The leaves of mango mistletoes were extracted using 96% ethanol solvent, and the extract was administered to aged female mice (18–20 months) orally with different doses for each group, namely 150, 300, and 600 mg/kg. Mango mistletoe leaves extract was administered once a day for 14 days. Then, the IL-2 levels of the mice were checked from their heart blood samples using Enzyme-Linked Immunosorbent Assay, while the percentages of CD4+CD28+ and CD8+CD28+ were examined from the spleen samples using flow cytometry. RESULTS: The ethanol extract of mango mistletoe leaves was able to increase the percentage of CD4+CD28+ significantly (p < 0.05) at doses of 300 and 600 mg/kg and increase the percentage of CD8+CD28+ significantly (p < 0.05) at a dose of 600 mg/kgBW, while other various doses had a strong enough correlation (r = 0.48) to increase IL-2 levels. CONCLUSION: The ethanol extract of mango mistletoe leaves has the good potential to inhibit the aging process in the immune system, as characterized by an increase in IL-2 levels and the percentage of CD4+CD28+ and CD8+CD28+. |
| Databáze: | OpenAIRE |
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