Assessing two different peroxidases´ potential for application in recalcitrant organic compound bioremediation
| Autor: | Nelson Caicedo, Edgar Gutiérrez, Rodrigo Torres |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Revista Colombiana de Biotecnología, Vol 3, Iss 2, Pp 66-77 (2011) |
| ISSN: | 1909-8758 0123-3475 |
| Popis: | This work shows the promising future presented by the following enzymes: Chloroperoxidase (CPO) from Caldariomyces fumago and royal palm peroxidase (Roystonea regia, PPR). These peroxidases were obtained from different sources (microbial and vegetable) and used as biocatalysts for applicating them in bioremediation of recalcitrant organic compounds. Each one of the enzymes' peroxidase catalytic activity was evaluated in organic phase systems, using different model compounds such as: PAHs (pyrene and anthracene), organic-nitrogenated compounds (diphenylamine), monoaromatic phenolic molecules (guayacol) and dyes (methyl orange and ABTS). The reaction systems were composed of mono-phase water mixtures and organic miscible solvent (methanol, ethanol, isopropanol, acetonitrile, tetrahydrofuran, dimethyl sulfoxide and dimethyl formamide), on which both peroxidases' catalytic activity was evaluated. The two enzymes' catalytic activity was observed on the evaluated substrates in most of these assays. However, PPR did not show biocatalytic oxidation for methyl orange dye and some PAHs. This enzyme did show the best tolerance to the evaluated solvents. Its catalytic activity was appreciably enhanced when low hydrophobic solvents were used. The kcat was calculated from this experimental data (as kinetic parameter) leading to each enzyme's biocatalytic performance on substrates being compared. |
| Databáze: | OpenAIRE |
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