| Autor: |
Mehdi Fazeli, Saeid Hosseinzadeh |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
International Journal of Fertility and Sterility, Vol 3, Iss 3, Pp 129-134 (2009) |
| ISSN: |
2008-0778 |
| Popis: |
Background: Chlamydia trachomatis is considered as an important cause of preventable sexuallytransmitted diseases, worldwide. It is known to be of an obligate intracellular nature and entersits target cells via an endocytic process. As major outer membrane protein (MOMP) is one of themain candidates for the attachment and entry of chlamydia to the host cells, we have tried to labelthe epitopes by using different techniques.Materials and Methods: McCoy cells were experimentally inoculated with 104 elementarybodies (EBs) followed by 24 hours incubation at 37ºC. The infected cells were then processedfor direct fluorescent antibody (DFA) and transmission electron microscopy (TEM) using anti-MOMP antibody and pre- and post-embedded labelling techniques.Results: DFA was able to detect 11/11 (100%) of the infected cells. These values were recordedas 9/11 (81.81%) and 8/11 (72.72%), using pre- and post-embedded techniques, respectively.Conclusion: MOMP is proposed to be one of the most important adhesion molecules forchlamydial attachment and entry into host cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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