Adventitious shoot regeneration from in vitro cultured leaves of guava (Psidium guava L.)
| Autor: | Óscar Concepción Laffitte, Lelurlys Nápoles Borrero, Aurora Pérez Martínez, Ninel Peralta Ballbé, Reinaldo Trujillo Sánchez |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Revista Colombiana de Biotecnología, Vol 6, Iss 2, Pp 54-61 (2007) |
| ISSN: | 1909-8758 0123-3475 |
| Popis: | Adventitious regeneration is a key step in the application of genetic engineering to the breeding programs of plants. In this work a method for adventitious shoot regeneration from leaves of micropropagated guava shoots has been developed and some of main factors to affect the shoot regeneration like, concentration of plant growth regulators (citoquinine), physiological state of explants and the wound are studied. Leaves from guava in vitro cultured of variety Cuban Red Dwarf 18-40 was used like explant in all experiments. The best re-sult was reached with MS basal medium supplemented with 0.75 mg/L of 6-benzylaminopurine. Was de-monstrated that for the leaves with more multiplication subculture number and taken from lower part of shoot (older); the morfogenetic potential falls significantly (p60%) and the largest number of shoot per regenerating leaf (>3) were obtained with several wounds carried out in f orm of jabs to the central nerve of leaves. This regeneration protocol constitutes an important tool that can be applied for future studies of genetic transformation in this species. Key words: Tissue culture, leaf explants, growth regulators, organogenesis, guava |
| Databáze: | OpenAIRE |
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