| Autor: |
Ling CHEN, Kang-jie LI, Yao HUANG, Zi-long CHEN, Rui ZHANG, Ge LI |
| Jazyk: |
čínština |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 44, Iss 9, Pp 739-744 (2019) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To investigate the effect of tobacco smoke exposure on the lower respiratory tract flora in mice. Methods Forty Kun-Ming male mice were randomly divided into smoking group and non-smoking group (20 each) according to their weight: In addition, in vitro group was set (n=20). Mice in smoking group were exposed to tobacco smoke 2 h/d for 90 days, and in non-smoking group and in vitro group were not treated. All mice were sacrificed 90 days later. Different concentrations of gradient cigarette smoke solutions (CSS) were configured by Mueller-Hinton Broth (MHB) step-by-step dilution method. The growth of lower respiratory tract flora was observed in mice of in vitro group by adding lung lavage. The pathological changes of lung tissues of mice in smoking group and non-smoking group were observed by hematoxylin-eosin staining. The development of lower respiratory tract flora in mice was detected by 16S rRNA sequencing. Results The minimum inhibitory concentration of CSS on the lower respiratory tract flora in mice of in vitro group was about 14.43 μg/ml. When CSS concentration was |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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