BREEDING OF MONOKARYOTICS GENITORS OF SYMBIOTIC FUNGUS Pisolithus microcarpus FOR GENETIC STUDIES

Autor: Nilza de Lima Pereira Sales, Sebastião Carlos da Silva Rosado, Dulcinéia de Carvalho, Anderson Marcos de Souza
Jazyk: angličtina
Rok vydání: 2003
Předmět:
Zdroj: Cerne, Vol 9, Iss 2, Pp 205-212 (2003)
ISSN: 0104-7760
Popis: This research developed a strategy for genetic improvement of theectomycorhizal f ungus Pisolithus microcarpus aiming at producing commercial innoculumfor Eucalyptus seedlings. After mapping the spatial and temporal distribution of fruitingbodies in two experimental plots of a Eucalyptus understory, basidiospores were collectedfrom six basidiomata and germinated to produce parental monokaryons isolates in vitro.After the identification of the sexual compatibility system of monokaryons, some compatiblesparental isolates were crossed in vitro to produce hybrid dikaryotic progenies and to developa model applicable to a diallell mating design. The diallell analyses were done for de trait ofmycelia growth. The use of such model allowed the estimation of the values of GeneralCombining Ability (GCA) of each parental monokaryons isolates and the values of SpecificCombining Ability (SCA), for each one of the crossing system between parentalmonokaryons. These combining abilities showed the potential use of basiospores to producecommercial innoculum.
Databáze: OpenAIRE