| Autor: |
Fang-ju SU, Wei-ze ZHANG, Ling MA, Fei WANG, Xiao-hua ZHAO, Xue-ling AO, Yan LIU |
| Jazyk: |
čínština |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 38, Iss 5, Pp 363-368 (2013) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To explore the expression of hypoxia-inducing factor-1α (HIF-1α) and its significance in the rats with isoproterenol-induced atrial fibrosis. Methods Thirty male Wistar rats were randomly divided into control group, isoproterenol [Iso, 5mg/(kg.d) for 7 days] group, and Iso + sirolimus (Rapa) group. The atrial fibrosis rat model was reproduced by subcutaneous injection of high doses of Iso in Iso group and Rapa group. Rats were treated with isoproterenol plus sirolimus in Iso-si group. All the animals were sacrificed 15 days after treatment. The contents of angiotensinⅡ(AngⅡ) in rat myocardial tissue were determined by radioimmunoassay. HE staining and Masson staining were performed for the measurement of the degree of atrial fibrosis in terms of collagen volume fraction (CVF). Immunohistochemistry and Western blotting were performed to detect the expressions of HIF-1α, TGF-β1 and MMP-9. The correlations of HIF-1α, TGF-β1 and MMP-9 to CVF and expressions of HIF-1α, TGF-β1 and MMP-9 were analyzed. Results The AngⅡ content was significantly higher in Iso group and Rapa group than that in control group (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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