Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni

Autor:	Vidigal Teofânia HDA, Montresor Lângia C, Simpson Andrew JG, Carvalho Omar S
Jazyk:	angličtina
Rok vydání:	2002
Předmět:	lcsh:Arctic medicine. Tropical medicine Biomphalaria lcsh:RC955-962 polymerase chain reaction fungi parasitic diseases lcsh:QR1-502 cytochrome oxidase I restriction fragment length polymorphism lcsh:Microbiology
Zdroj:	Memórias do Instituto Oswaldo Cruz., Vol 97, Iss suppl.1, Pp 47-52 (2002)
ISSN:	1678-8060 0074-0276
Popis:	The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed to be the most informative once they were polymorphic patterns, corroborating with much morphological data. In addition, we performed COI digestion of B. straminea snails from Uruguay and Argentina.
Databáze:	OpenAIRE
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