Phytochemical investigation of Crataegi folium cum flos (hawthorn leaves and flowers) and Hyperici herba (st johns wort aerial parts) hydroalcoholic extracts

Autor: Ruxandra Cretu, Roxana Mihailescu, Gabriela Mitroi, Elena Iacob, Ramona Verdes, Carmen Tebrencu
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara, Vol 12, Iss 4, Pp 133-138 (2011)
ISSN: 2248-3276
1582-3571
Popis: Hawthorn (Crataegus monogyna) hydroalcoholic extract was prepared by extraction of powdered dried leaves and flowers with ethanol 70% v/v (1:10), by reflux for two hours. St Johns Wort (Hypericum perforatum) hydroalcoholic extract was prepared by extraction of powdered dried flowering aerial parts with ethanol 70% v/v (1:10), by reflux for two hours. Both extracts were qualitative and quantitative analyzed. The qualitative analysis consisted in phytochemical screening and spectroanalytical profile by HPTLC and UV/VIS absorption spectroscopy. Quantitative analyses consisted in determination of total flavonoid content (as rutin) by following colorimetric aluminum chloride method, and total polyphenol content (as gallic acid) by Folin- Ciocalteu method. Results were evaluated statistically and presented as mean of three determinations ± SD (standard deviation). These analyses revealed their complex composition and either some similarities and differences between these two extracts.
Databáze: OpenAIRE