| Popis: |
The pig-raising model with pig-on-litter system is novel and environmentally friend. The present paper dealt with biocontrol ef-fects of pig-on-litter system on the pig bacterial pathogens. The community dynamics of bacteria, fungi, actinomycetes were observed and the indicators of Escherichia coli and Salmonella enterica were isolated to clear the biocontrol effects of pig-on-litter system. Bacteria, fungi and actinomycetes from the four layers in the different periods of the stroma cushion were isolated by NA, PDA and Gause's No.1 media, E.coli was separated by the method of eosin methylene blue(EMB)medium and S. enterica was isolated by the bismuth sulfite agar(BS)medium, indicating the microbiological community dynamic with biocontrol effects of pig-on litter system on the pig bacterial pathogens. The results showed that bacteria was the dominant, the distribution number reached 107cfu·g-1. The community dynamics of bacteria first increased and then decreased. Compared with bacteria, the distribution of fungi and actinomycetes in the low number with 3~4 magnitude, and gradually decreased as the time increased. The amount of E. coli and S. enterica in the stoma cushion were significantly negatively correlated with the bacteria, positively correlated with fungi and actinomycetes. The pig-on-litter system could inhibit the growth of E. coli and S. enterica. The amount of E. coli and S. enterica were much more less in the five-months stroma cushion than that in the one-month stroma cushion with de-creasing rates of 95.34% and 44.41% respectively, revealed that the pig-on-litter system could play an important role on biogicontrol of E. coli and S. enterica in the piggery. |
