| Popis: |
Liquid-liquid extraction is one of the most widely used techniques in various sectors of the biotechnology industries. This technique has found limited application in particular for the separation, concentration and purification of enzymes. The main reason for this is the lack of appropriate solvents with the desired selectivity and the solvents which does not provide the biocompatible environment for the enzymes of interest. However, the organic solvents do not satisfy the selectivity or the preservation of the enzyme activity, structure and its functional properties. Therefore, this necessitates the development of efficient and inexpensive technique, to obtain the high activity, purity and recovery of the enzymes. Reverse micellar technique was found to be safe media in biotechnology industry, because the reverse micellar core avoids the contact between enzymes and solvents. As well as this technique provides compatible surroundings, selectivity, and safeguard the functional properties, structure and activity of the enzymes. In this review we described the concept of reverse micelles, there compositions and the steps involved in extraction of enzymes using reverse micelles method. Also, it highlights the current progress/recent developments in reverse micellar extraction for the improvement of technique by exploiting the reverse micelle phenomenon. |
