| Autor: |
Rui CHEN, Xiao-gang GAO, Lei ZHANG, Xiao-min SHI, Wen-yuan GUO, You-hua ZHU, Zhi-ren FU |
| Jazyk: |
čínština |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 42, Iss 6, Pp 515-519 (2017) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To observe whether treatment with cannabidiol (CBD) affect nonalcoholic steatohepatitis (NASH) induced by methionine choline-deficient diet (MCD) in rats and investigate the underlying molecular mechanism. Methods Sixty-three adult male SD rats were randomly divided in to three groups as follows: Control group (rats fed with normal diet), MCD group (rats fed with MCD and MCD+CBD group [rats fed with MCD and treated with cannabidiol, 2mg/(kg.d), i.p.]. Ten weeks later, steatohepatitis and fibrosis were evaluated by hematoxylin-eosin and Masson staining, respectively. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by using an automatic biochemical analyzer and hepatic levels of cholesterol and triacylglycerol were determined with kits. Autophagic flux in livers was evaluated by Western blotting. Results Treatment with cannabidiol reduced ratio of liver/body weightratio (4.2%±0.6% versus 3.1%±0.6%, P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
