PREVALENCE OF PATHOGENIC GENES OF CAMPYLOBACTER JEJUNI AND CAMPYLOBACTER COLI ISOLATED FROM ENVIRONMENTAL MATERIAL
| Autor: | Małgorzata Andrzejewska, Bernadeta Szczepańska, Dorota Śpica, Jacek J. Klawe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Medycyna Środowiskowa, Vol 13, Iss 2, Pp 57-62 (2010) |
| ISSN: | 2084-6312 1505-7054 |
| Popis: | li are recognized as a leading bacterial cause of foodborne disease in developed countries. Among raw materials of animal origin poultry is the main carrier of Campylobacter. Other important source is untreated water. Recently, some genes have been recognized as responsible for the expression of pathogenicity: flaA gene (motility), cadF (adhesion), cdtB (toxin production), iam sequence (invasion). Material and methods: The materials to investigate were 63 Campylobacter spp. strains isolated from chickens carcasses, obtained in supermarkets in Bydgoszcz (22 C. jejuni, 41 C. coli) and 37 Campylobacter spp. strains (17 C. jejuni, 20 C. coli) from surface water samples (ponds, Brda river). The presence of the flaA, cadF, cdtB genes and iam sequence was determined with the PCR method with specific primers. Results: All of the Campylobacter isolates from water samples carried the cadF gene. The cadF and flaA genes were detected in 100% of C. jejuni strains from poultry. The high prevalence of the cdtB gene and iam sequence, associated with invasion and toxin production were observed in this study. More than 80% of isolates from poultry possessed these genes. Lower prevalence (67,5% cdtB, 75,6% iam) was observed in water samples isolates. Conclusion: 1. High prevalence of Campylobacter pathogenic genes indicate an important for the pathogenesis process role of these genes. 2. Poultry and water from examine water basins are source of invasive and pathogenic C. jejuni and C. coli strains and could be a reason of campylobacteriosis in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|