| Autor: |
Shi HU, Ya-feng SHEN, Tian LI, Chang-hai LEI |
| Jazyk: |
čínština |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 42, Iss 7, Pp 612-616 (2017) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To prepare human anti-postsynaptic neurotoxin monoclonal antibody from phage antibody library using recombined postsynaptic short-chain neurotoxins of Lapemis curtus. Methods The three postsynaptic neurotoxins were expressed in Escherichia coli and phage antibodies against neurotoxins were screened. The obtained scFvs were further constructed to full antibodies. The antigen binding ability, biochemical and pharmacokinetic characteristics of the depurated antibodies were evaluated, and the anti-toxin effects of the antibody drugs were verified in vivo. Results Two positive scFvs with specific binding ability to all the three neurotoxins were obtained after 4 rounds of panning, and then the full antibodies were generated, expressed and purified. Antibody binding specificity was further confirmed. Pharmacokinetics of these two antibodies, SM-SD-911 and SM- SD-861 were similar to a conventional IgG molecule. SM-SD-911 and SM-SD-861 also showed strong antitoxin effect in vivo. Conclusion full human anti-postsynaptic neurotoxin antibody has been successfully obtained by using recombinant neurotoxin technology and a large phage antibody library which may achieve clinical efficacy in navy medical applications. DOI: 10.11855/j.issn.0577-7402.2017.07.07 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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