| Popis: |
A rapid quantitative detection of urinary RBP was established by using nano-gold immunochromatography (sandwich method) and trisodium citrate reduction method and a rapid immunochromatographic test strip was developed. Theimmunochromatographic test strip can quantitatively detect RBP within 15 minutes. The detection limit was 150ng/mL and detection range was from 150 to 5000 ng/mL. There were no cross-reactions with others kidney disease markers,such as urinary albumin (ALB),transferrin protein (TRF),β2-microglobulin (β2-MG),urinary fiber connecting protein (FN),and lysozyme (LZM). The results indicate that it is a quick and simple method with strong specificity,high sensitivity,and wide detection range. The rapid detection method will have extensive clinical applications in the early diagnosis of proximal tubular damage,kidney disease,diabetic nephropathy,and process monitoring. |
