| Autor: |
Ye ZHANG, Lian-yang ZHANG, Shi-jin SUN, Hao TAN, Yang LI |
| Jazyk: |
čínština |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 41, Iss 1, Pp 22-25 (2016) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To investigate different subcellular distribution and the effect on cell permeability of vascular endothelial cadherin (VE-Cad) in cytoplasm via clathrin-and caveolae-mediated endocytosis pathways after lipopolysaccharide (LPS) challenge. Methods The co-immunoprecipitation of VE-Cad with Rab11 (marker of the recycling endosomes) and lysosome-associated membrane protein 2 (LAMP2, marker of the late endosomes/lysosomes) at different time points, as well as the effects of clathrin-mediated endocytosis inhibitor chlorpromazine (CPZ) and caveolae inhibitor filipin on the coimmunoprecipitation of VE-Cad with Rab11/LAMP2 and on the monolayer cell permeability after LPS(10µg/ml) treatment, were observed. Results 1) The co-immunoprecipitation of VE-Cad with Rab11 was increased at 1h after LPS treatment (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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